EcoCyc is a comprehensive, literature-curated bioinformatics database for the model bacterium Escherichia coli K-12 MG1655, detailing its complete genome, genes, proteins, metabolic pathways, transport systems, and transcriptional regulation to understand the cell's molecular workings. It serves as an electronic reference for biologists, offering tools for omics data analysis (like gene expression), comparative genomics, and metabolic modeling, accessible via a web interface. 

MetaCyc is a comprehensive, curated database of experimentally verified metabolic pathways, enzymes, and metabolites from all domains of life, used for understanding metabolism in various organisms, supporting metabolic engineering, drug discovery, and genome analysis through its interactive diagrams, search tools, and links to external biological databases. It catalogues a diverse range of pathways, from core functions to secondary metabolism, providing rich data for researchers. MetaCyc transitioned to a subscription-only model for full access as of January 2024, meaning paid BioCyc subscriptions are needed for deeper institutional access or downloadable versions, though basic browsing remains free. WCM does no longer subscribed to BioCyc.